WORKS™ Optimization Procedures The Optimization Procedure provides a detailed protocol and data collection instructions to optimize SmartFlow™ TFF based separations. Systematic evaluations of membranes, process conditions, and sample make-up are featured.
WORKS Case Study The Case Study provides examples of how others have applied the principles of the Optimization Procedure to their specific application.
WORKS Protocol Where appropriate, NCSRT has developed a simple protocol to address specific applications. Protocols provide exact instructions including membrane, pore size, filter area, and shear to achieve >90% yield in most cases. The protocol is not optimized for any individual product, but for classes of products like Mabs, phage, and whole cells. Designed to provide acceptable results for over 80% of those who try it, the protocol is an excellent way to jump start the improvement in separation efficiency while optimizing the SmartFlow filter process.

Products:

AT WORKS Services:

WORKS WORKbooks:

• Isolation of secreted proteins from mammalian cell lines
  
• Isolation of small molecules
  
• Simultaneous isolation and concentration
  
• Ultrafiltration, concentration, and diafiltration
  
• Secreted proteins from whole cells
  
• Isolation of proteins from cell lysate
  
• Concentration and diafiltration of whole cells
  
• Purification of secreted viral antigens from mammalian cells
  
• Viral antigen purification by diafiltration
  
• Isolation of bacterial phage
  
• Purification of viral antigens by continuous diafiltration
  
• Isolation of a secreted protein by continuous cell diafiltration
  
• Concentration and diafiltration of viral antigens
  

Procedures:

• SmartFlow™ CIP procedure

Worksheets

• Membrane Test Worksheet